Long-term culture, genetic manipulation and xenotransplantation of human normal and breast cancer organoids

Hans Clevers, Johanna F. Dekkers, Esmée J. van Vliet, Norman Sachs, Jennifer M. Rosenbluth, Oded Kopper, Heggert G. Rebel, Ellen J. Wehrens, Carol Piani, Jane E. Visvader, Carla S. Verissimo, Sylvia F. Boj, Joan S. Brugge, Anne C. Rios

Published: 2021-03-09 DOI: 10.1038/s41596-020-00474-1

xenotransplantation

breast cancer organoids

long-term culture

genetic manipulation

orthotopic transplantation

AI 解读

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Organoid technology has revolutionized the study of human organ development, disease and therapy response tailored to the individual. Although detailed protocols are available for the generation and long-term propagation of human organoids from various or
Introduction
Materials
Procedure
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Timing
Anticipated results
- Reporting Summary
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References
ACKNOWLEDGMENTS

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Long-term culture, genetic manipulation and xenotransplantation of human normal and breast cancer organoids

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